Granomar

A workbench for plant stress granules: from seeds on a plate to granules in a cell, to the RNAs and proteins inside them, and the papers that came before.

Each section turns raw lab files into measured results, explains every step in plain words and ends with a PDF report and the tables.

Stress Granule Lab of Prof. Monika Chodasiewicz·King Abdullah University of Science and Technology (KAUST)
With thanks to Israel for supervision, and to the members of the Stress Granule Lab.
Root tip, no heat
Root tip, 42 °C for 1 h
Root tip, 42 °C for 6 h
Root tip, recovery 6 h
No heatcontrol root
42 °C, 1 hfoci form
42 °C, 6 hcontinuous heat
Recovery, 6 hafter 1 h at 42 °C
Heat stress granules form and clear. RNA foci (FAM-labelled RNA) in Arabidopsis root tips from the lab's heat time course; 40 µm fields, scale bar 10 µm. The control root was imaged on a different microscope.

From plate to paper

One stress granule story, five places to look
  1. Seeds on a plateGermination over time, line by line
  2. Granules in a cellFoci counted in root tips, under heat and after
  3. RNAs under stressWhich genes go up and down with heat
  4. Proteins inside granulesGranule members, found and predicted
  5. Papers before usThe plant stress granule literature in one map